Tag: Polymerase chain reaction

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COVID-19 Test Results: Don't Discount Clinical Intuition

Newsemia
Accurate diagnosis of COVID-19 may require clinical intuition as COVID-19 tests prove less reliable than desired and SARS-CoV-2 turns out to be more wily than...
Biology

Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing

Newsemia
by Povilas Gibas, Milda Narmontė, Zdislav Staševskij, Juozas Gordevičius, Saulius Klimašauskas, Edita Kriukienė 5-hydroxymethylcytosine (5hmC) is the most prevalent intermediate on the oxidative DNA demethylation...
Biology

ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

Newsemia
by Jelmer Willems, Arthur P. H. de Jong, Nicky Scheefhals, Eline Mertens, Lisa A. E. Catsburg, Rogier B. Poorthuis, Fred de Winter, Joost Verhaagen, Frank...
Biology

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae

Newsemia
by Nathan G. Schoepp, Eric J. Liaw, Alexander Winnett, Emily S. Savela, Omai B. Garner, Rustem F. Ismagilov The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections...
Biology

Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic β-lactam antibiotic susceptibility testing of <i>Neisseria gonorrhoeae</i>

Newsemia
by Emily S. Savela, Nathan G. Schoepp, Matthew M. Cooper, Justin C. Rolando, Jeffrey D. Klausner, Olusegun O. Soge, Rustem F. Ismagilov Rapid antibiotic susceptibility...
Biology

Hypothalamic miR-30 regulates puberty onset via repression of the puberty-suppressing factor, Mkrn3

Newsemia
by Violeta Heras, Susana Sangiao-Alvarellos, Maria Manfredi-Lozano, María J. Sanchez-Tapia, Francisco Ruiz-Pino, Juan Roa, Maribel Lara-Chica, Rosario Morrugares-Carmona, Nathalie Jouy, Ana P. Abreu, Vincent Prevot,...
Biology

Sex chromosomes control vertical transmission of feminizing <i>Wolbachia</i> symbionts in an isopod

Newsemia
by Thomas Becking, Mohamed Amine Chebbi, Isabelle Giraud, Bouziane Moumen, Tiffany Laverré, Yves Caubet, Jean Peccoud, Clément Gilbert, Richard Cordaux Microbial endosymbiosis is widespread in...
Biology

EXP1 is critical for nutrient uptake across the parasitophorous vacuole membrane of malaria parasites

Newsemia
by Paolo Mesén-Ramírez, Bärbel Bergmann, Thuy Tuyen Tran, Matthias Garten, Jan Stäcker, Isabel Naranjo-Prado, Katharina Höhn, Joshua Zimmerberg, Tobias Spielmann Intracellular malaria parasites grow in...
Biology

Intestinal delta-6-desaturase activity determines host range for <i>Toxoplasma</i> sexual reproduction

Newsemia
by Bruno Martorelli Di Genova, Sarah K. Wilson, J. P. Dubey, Laura J. Knoll Many eukaryotic microbes have complex life cycles that include both sexual...
Biology

Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in <i>Escherichia coli</i>

Newsemia
by Matthew Cummings, Anna D. Peters, George F. S. Whitehead, Binuraj R. K. Menon, Jason Micklefield, Simon J. Webb, Eriko Takano Polyketides are a class...
Biology

Global proteomic analyses define an environmentally contingent Hsp90 interactome and reveal chaperone-dependent regulation of stress granule proteins and the R2TP complex in a fungal pathogen

Newsemia
by Teresa R. O’Meara, Matthew J. O’Meara, Elizabeth J. Polvi, M. Reza Pourhaghighi, Sean D. Liston, Zhen-Yuan Lin, Amanda O. Veri, Andrew Emili, Anne-Claude Gingras,...
Biology

A natural histone H2A variant lacking the Bub1 phosphorylation site and regulated depletion of centromeric histone CENP-A foster evolvability in <i>Candida albicans</i>

Newsemia
by Cedric A. Brimacombe, Jordan E. Burke, Jahan-Yar Parsa, Sandra Catania, Teresa R. O’Meara, Jessica N. Witchley, Laura S. Burrack, Hiten D. Madhani, Suzanne M....
Biology

mRNA association by aminoacyl tRNA synthetase occurs at a putative anticodon mimic and autoregulates translation in response to tRNA levels

Newsemia
by Ofri Levi, Yoav Arava Aminoacyl-tRNA synthetases (aaRSs) are well studied for their role in binding and charging tRNAs with cognate amino acids. Recent RNA...

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