Tag : Plasmid construction

Biology

A compact Cas9 ortholog from <i>Staphylococcus Auricularis</i> (SauriCas9) expands the DNA targeting scope

Newsemia
by Ziying Hu, Shuai Wang, Chengdong Zhang, Ning Gao, Miaomiao Li, Deqian Wang, Daqi Wang, Dong Liu, Huihui Liu, Sang-Ging Ong, Hongyan Wang, Yongming Wang...
Biology

Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase

Newsemia
by Iker Lamas, Laura Merlini, Aleksandar Vještica, Vincent Vincenzetti, Sophie G. Martin Local activity of the small GTPase Cdc42 is critical for cell polarization. Whereas...
Biology

Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in <i>Escherichia coli</i>

Newsemia
by Matthew Cummings, Anna D. Peters, George F. S. Whitehead, Binuraj R. K. Menon, Jason Micklefield, Simon J. Webb, Eriko Takano Polyketides are a class...
Biology

Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1

Newsemia
by Tong Liu, Tomomi Kuwana, Hongkai Zhang, Matthew G. Vander Heiden, Richard A. Lerner, Donald D. Newmeyer Bcl-2 family proteins control a decisive apoptotic event:...
Biology

Comprehensive profiling of the STE20 kinase family defines features essential for selective substrate targeting and signaling output

Newsemia
by Chad J. Miller, Hua Jane Lou, Craig Simpson, Bert van de Kooij, Byung Hak Ha, Oriana S. Fisher, Natasha L. Pirman, Titus J. Boggon,...
Biology

Environment-induced same-sex mating in the yeast <i>Candida albicans</i> through the Hsf1–Hsp90 pathway

Newsemia
by Guobo Guan, Li Tao, Huizhen Yue, Weihong Liang, Jiao Gong, Jian Bing, Qiushi Zheng, Amanda O. Veri, Shuru Fan, Nicole Robbins, Leah E. Cowen,...
Biology

Centromeric signaling proteins boost G1 cyclin degradation and modulate cell size in budding yeast

Newsemia
by Joan M. Martínez-Láinez, David F. Moreno, Eva Parisi, Josep Clotet, Martí Aldea Cell size scales with ploidy in a great range of eukaryotes, but...

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