Determination of Oroxylin A, Oroxylin A 7-O-glucuronide, and Oroxylin A sodium sulfonate in beagle dogs via ultra-high-performance liquid chromatography/tandem mass spectrometry: Application in a pharmacokinetic study.
J Sep Sci. 2020 Mar 18;:
Authors: Ren G, Chen H, Zhang M, Yang N, Yang H, Xu C, Li J, Ning C, Song Z, Zhou S, Zhang S, Wang X, Lu Y, Li N, Zhang Y, Chen X, Zhao D
Oroxylin A, obtained from the root of Scutellaria baicalensis Georgi, is a flavonoid with anti-tumor and other pharmacological activities. Our previous studies showed for the first time that it is mainly metabolized to Oroxylin A sodium sulfonate by sulfotransferase enzymes in beagle dogs. In this study, rapid, universal, selective, and robustly ultra-high performance liquid chromatography/tandem mass spectrometry methods were established and fully validated to quantitatively detect Oroxylin A, Oroxylin A 7-O-glucuronide, and Oroxylin A sodium sulfonate in beagle dog plasma. The quantitative analysis for Oroxylin A sodium sulfonate was reported for the first time. Plasma samples were processed with acetonitrile, a universal protein precipitant. Gradient elution was performed to resolve carryover effects and to achieve separation efficiency and sufficient chromatographic retention. The linear relationships of Oroxylin A, Oroxylin A 7-O-glucuronide, and Oroxylin A sodium sulfonate in plasma were in the range of 2.0∼500.0 ng/mL, 5.0∼500.0 ng/mL, and 1.881∼940.5 ng/mL, respectively. The assay method was successfully applied to pharmacokinetic study. This is the first paper that reveals the pharmacokinetic profile of Oroxylin A, Oroxylin A 7-O-glucuronide, and Oroxylin A sodium sulfonate after single-dose intravenous and oral administration of Oroxylin A in beagle dogs. This article is protected by copyright. All rights reserved.
PMID: 32187438 [PubMed – as supplied by publisher]