Biology

Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult <i>Drosophila</i>



by Leslie Ann Jaramillo Koyama, Andrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B. Ludington, Kerwyn Casey Huang, Lucy Erin O’Brien

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount’s capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.

Source link




Related posts

Questionomics: Using Big Data to Ask and Answer Big Questions

Newsemia

University of Georgia: A Tenure-Track Assistant Professor of Environmental Health Science

Newsemia

Fluid shift vs. body size: changes of hematological parameters and body fluid volumes in hindlimb-unloaded mice, rats and rabbits [RESEARCH ARTICLE]

Newsemia

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Accept Read More

Privacy & Cookies Policy