Biology

Water and ion transport across the eversible vesicles in the collophore of a springtail Orchesella cincta [RESEARCH ARTICLE]

Barbora Konopova, Dennis Kolosov, and Michael J. O’Donnell

Springtails (Collembola) are ancient close relatives of the insects. The eversible vesicles are their unique paired transporting organs, which consist of an epithelium located inside a tube-like structure called the collophore on the first abdominal segment. The vesicles can be protruded out of the collophore and several lines of evidence indicate that they have a vital function in water uptake and ion balance. However, the amount of water absorbed by the vesicles and which other ions apart from sodium are transported remain unknown. Using Orchesella cincta as a model, we developed protocols for two assays that enabled us to study water and ion movement across the eversible vesicles in whole living springtails. Using an inverse Ramsay assay we demonstrate that the eversible vesicles absorb water from a droplet applied onto their surface. Using the scanning ion-selective electrode technique (SIET) we show that the vesicles absorb Na+ and Cl from the bathing medium, secrete NH4+, and both absorb and secrete K+. H+ is secreted at a low level in the anterior part and absorbed at the posterior part. We did not detect transport of Ca2+ at significant levels. The highest flux was the absorption of Cl, and the magnitude of ion fluxes were significantly lower in fully hydrated springtails. Our data demonstrate that the eversible vesicles are a transporting epithelium functioning in osmo- and ionoregulation, nitrogenous waste excretion and likely acid-base balance.

Source link




Related posts

Does the left aorta provide proton-rich blood to the gut when crocodilians digest a meal? [SHORT COMMUNICATION]

Newsemia

Effects of environmental enrichment on forebrain neural plasticity and survival success of stocked Atlantic salmon [RESEARCH ARTICLE]

Newsemia

Erratum for Sehrawat et al., "Cancer-Associated Eukaryotic Translation Initiation Factor 1A Mutants Impair Rps3 and Rps10 Binding and Enhance Scanning of Cell Cycle Genes" [Errata]

Newsemia

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Accept Read More

Privacy & Cookies Policy