A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka

by Amaury Herpin, Cornelia Schmidt, Susanne Kneitz, Clara Gobé, Martina Regensburger, Aurélie Le Cam, Jérome Montfort, Mateus C. Adolfi, Christina Lillesaar, Dagmar Wilhelm, Michael Kraeussling, Brigitte Mourot, Béatrice Porcon, Maëlle Pannetier, Eric Pailhoux, Laurence Ettwiller, Dirk Dolle, Yann Guiguen, Manfred Schartl

Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1—acting as master sex-determining gene—has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3′ UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans—together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells—suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.

Source link

Related posts

New HIV vaccine could expose latent virus and kill it


Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies


NYU Institute for Systems Genetics: Postdoctoral Fellow in Regulatory Genomics


This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Accept Read More

Privacy & Cookies Policy